Single-Cell RNA Sequencing with PIPseq Chemistry: A Scalable, Accessible Workflow​ @IlluminaInc
Single-Cell RNA Sequencing with PIPseq Chemistry: A Scalable, Accessible Workflow​  @IlluminaInc
Uploaded July 2026 | Updated September 2026, 3 weeks ago
Single-cell RNA sequencing provides a high-resolution view of gene expression within complex biological systems. Illumina Single Cell 3′
RNA Prep, powered by PIPseq (particle-templated instant partition sequencing) chemistry, enables a scalable and accessible approach to
single-cell analysis.​

This workflow demonstrates how researchers can perform single-cell RNA sequencing using a flexible, microfluidics-free method that
simplifies implementation while maintaining high data quality.​

Key features of the workflow include:​
- Microfluidics-free sample preparation using standard laboratory equipment​
- Scalable from small pilot studies to high-throughput experiments ​
- 3′ transcriptome profiling for sensitive gene expression analysis ​
- Consistent, reproducible results enabled by PIPseq chemistry ​
- Seamless integration with Illumina sequencing platforms and downstream analysis with Illumina Connected Multiomics​

By reducing workflow complexity and enabling flexible study design, this approach supports both new and experienced researchers in
expanding single-cell RNA sequencing applications.​

For Research Use Only. Not for use in diagnostic procedures



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Single-Cell RNA Sequencing with PIPseq Chemistry: A Scalable, Accessible Workflow​

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