Uploaded August 2023 | Updated September 2026, 1 hour ago
In this installment of PyMOL 101, KP talks about CPK coloring and color settings in PyMOL. We’ll learn to change renderings, color them, and apply transparency.
00:00 Intro AND CPK coloring
00:40 Phosphofructokinase background
01:24 Setting up your workspace
02:06 Color by chain
03:23 Color by secondary structure
03:52 Surface transparency
04:51 Color by element
06:05 Terminology break
06:28 Rendering a ligand
07:26 Cartoon transparency
08:14 Overview of the different renderings
REFERENCES:
• proteopedia.org/wiki/index.php/CPK#:~:text=CPK%20Color%20Scheme%20for%20the,yellow%2C%20and%20hydrogen%20is%20white.
• PDB ID: 6PFK
• Schirmer, T., Evans, P. Structural basis of the allosteric behaviour of phosphofructokinase. Nature 343, 140–145 (1990). doi.org/10.1038/343140a0
In this installment of PyMOL 101, KP talks about CPK coloring and color settings in PyMOL. We’ll learn to change renderings, color them, and apply transparency.
00:00 Intro AND CPK coloring
00:40 Phosphofructokinase background
01:24 Setting up your workspace
02:06 Color by chain
03:23 Color by secondary structure
03:52 Surface transparency
04:51 Color by element
06:05 Terminology break
06:28 Rendering a ligand
07:26 Cartoon transparency
08:14 Overview of the different renderings
REFERENCES:
• proteopedia.org/wiki/index.php/CPK#:~:text=CPK%20Color%20Scheme%20for%20the,yellow%2C%20and%20hydrogen%20is%20white.
• PDB ID: 6PFK
• Schirmer, T., Evans, P. Structural basis of the allosteric behaviour of phosphofructokinase. Nature 343, 140–145 (1990). doi.org/10.1038/343140a0









![PyMOL 101, Lesson 3: File Types and Saving – Publication Quality Figures & Scenes
In this lesson, KP takes you through the input and output PyMOL files, how to save your session, how to export a high-quality PNG image. Finally, we’ll learn to save scenes, preserving the different renderings and views as you work through this tutorial.
00:00 Intro
00:21 PDB and CIF files
01:41 Publication quality image guidelines and saving
04:41 Saving scenes in a PyMOL session
REFERENCES
• Rachel Kramer Green, Beginner’s Guide to PDB Structures and the PDBx/mmCIF Format https://pdb101.rcsb.org/learn/guide-to-understanding-pdb-data/beginner%E2%80%99s-guide-to-pdb-structures-and-the-pdbx-mmcif-format
• PDB ID: 3uyn
• Elder, I., Fisher, Z., Laipis, P. J., Tu, C., McKenna, R., & Silverman, D. N. (2007). Structural and kinetic analysis of proton shuttle residues in the active site of human carbonic anhydrase III. PROTEINS: Structure, Function, and Bioinformatics, 68(1), 337-343.
***COMMAND LINE CODE to recreate the Lesson 2 structure***
fetch 3UYN, type=pdb1, multiplex=1
select zn, resn zinc
sele active, resi 5+7+62+64+67+94+96+119+199+200]
show sticks, active
zoom active
select active_water, ((resi 5+7+62+64+67+94+96+119+199+200, resn Zn)around 3.3) and(resn HOH)
hide nonbonded
show spheres, active_water
alter active_water, vdw=0.5
rebuild
hide sticks, name c+o+n PyMOL 101, Lesson 3: File Types and Saving – Publication Quality Figures & Scenes](https://i.ytimg.com/vi/ymkw7UMUnBw/mqdefault.jpg)
