Uploaded April 2011 | Updated September 2026, 3 weeks ago
Bio Rad's pGLO transformation kit is demonstrated in this video. The pGLO plasmid has 3 genes spliced into it. Bla is a gene that codes for a protein that nullifies the antibiotic ampicillin. Ara C is a gene that codes for a protein that turns on the GFP gene. The GFP gene codes for the green fluorescence protein (GFP), which glows green in the presence of UV light. The GFP is normally found in the jellyfish Aequorea victoria, which makes it bioluminescent. The pGLO plasmid is inserted into E. coli bacteria, which makes the bacteria survive in ampicillin and glow green in UV light.
ScienceFix.com
Producer: Darren Fix
Bio Rad's pGLO transformation kit is demonstrated in this video. The pGLO plasmid has 3 genes spliced into it. Bla is a gene that codes for a protein that nullifies the antibiotic ampicillin. Ara C is a gene that codes for a protein that turns on the GFP gene. The GFP gene codes for the green fluorescence protein (GFP), which glows green in the presence of UV light. The GFP is normally found in the jellyfish Aequorea victoria, which makes it bioluminescent. The pGLO plasmid is inserted into E. coli bacteria, which makes the bacteria survive in ampicillin and glow green in UV light.
ScienceFix.com
Producer: Darren Fix
