Uploaded September 2023 | Updated September 2026, 6 hours ago

---

Description: STEMCELL’s portfolio of TeSR™ 3D-optimized media products have been developed for robust and scalable suspension culture of human pluripotent stem cells (hPSCs) in aggregates. The workflows include non-enzymatic dissociation using the Gentle Cell Dissociation Reagent and filter-based trituration. For larger-scale hPSC suspension cultures, STEMCELL recommends the PBS-MINI Bioreactor with 0.1 L and 0.5 L vessels. Watch this technical video to learn how to passage a 100 mL hPSC suspension culture in a PBS-MINI Bioreactor 0.1 L vessel. stemcell.com/pbs-mini-magdrive-bioreactor.html

Timecode:
0:00 Intro
0:21 Preparing required reagents and materials
1:41 Imaging an aggregate sample to evaluate morphology
2:50 Clump passaging a 3D hPSC culture using GCDR and filter-based trituration
12:50 Seeding hPSC clumps into a PBS-MINI Bioreactor 0.1 L vessel

For a full list of products, as well as educational resources, visit our website: stemcell.com

Follow us on Twitter: bit.ly/3jW20Gv
Follow us on LinkedIn: bit.ly/32uKovJ
Follow us on Instagram: bit.ly/3LrNkOs
Like us on Facebook: bit.ly/32ern07
Subscribe to our YouTube channel: bit.ly/32f28ei