Long-read sequencing technologies for the generation of reference-level genome assemblies at scale @PacificBiosciences
Long-read sequencing technologies for the generation of reference-level genome assemblies at scale  @PacificBiosciences
Uploaded January 2025 | Updated September 2026, 7 hours ago
Dr. Caroline Howard from The Wellcome Sanger Institute discusses the Tree of Life programme, which aims to assemble reference-level genome assemblies for eukaryotic species. The programme's main project, the Darwin Tree of Life, seeks to sequence 70,000 species from Britain and Ireland. Additionally, the research team participates in the aquatic symbiosis genomics project, the Vertebrate Genomes Project, and the European Reference Genome Atlas, all under the Earth BioGenome Project. The programme has sequenced approximately 5,500 species and has numerous standardized protocols for DNA and RNA extraction, emphasizing their high-throughput processes achieved using the Revio system.

Dr. Howard further highlighted the challenges with specific organisms and the need for specialist knowledge in handling various species. Open Access protocols and continuous methodological evolution are key to high success rates in genome assembly.

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Long-read sequencing technologies for the generation of reference-level genome assemblies at scale

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