Uploaded May 2025 | Updated September 2026, 1 week ago
This video provides a complete step-by-step walkthrough for cleanroom training and operating the S-4800 Scanning Electron Microscope (SEM). It is for students, researchers, and first-time users.
Key points covered include:
1. Cleanroom Preparation to Prepare for and ALWAYS follow:
• Wear a lab coat, gloves, shoe covers, and eye protection (all provided in the lab).
• Follow cleanroom behavior protocols to maintain a contaminant-free environment.
• Always leave the microscopy laboratory in a nicer (cleaner) state than it was when you arrived.
2. Entering the Cleanroom
• Properly use and dispose of shoe covers.
• Move through clean zones to prevent particle contamination.
• If you have very messy shoes, leave them outside.
3. Filling the Cold Trap with Liquid Nitrogen
• Demonstration of correct filling procedure.
• Listen for the change in sound to know when it’s full—avoid overfilling. About 0.5 L is needed.
• Safety first: don’t pour liquid nitrogen on the machine or on yourself.
• Always use eye protection when working with liquid nitrogen.
4. Starting the SEM System
• Power is always on for the 4800 SEM, and check system status (e.g., idle mode).
• No need to start the microscope.
5. Using the Internal Camera
• Use the built-in camera for accurate sample positioning.
• Move slowly and carefully to avoid mistakes and dropping your sample inside the vacuum chamber.
6. Handling and Inserting Samples
• Clean sample handling techniques.
• Insert samples gently to protect the instrument and sample.
• More information on this in the next video.
7. Filling Out the Logbook
• Log your session start time, user name, and any relevant notes.
• Logging is mandatory: it helps staff track system use and plan maintenance.
• The SEM is not considered “in use” unless it’s properly logged.
• The SEM is booked (reserved via the LIMS system).
#sem #microscope #imaging
8. Why It Matters
• Ensures high-quality SEM imaging.
• Extends instrument lifetime and reduces repair costs
This video provides a complete step-by-step walkthrough for cleanroom training and operating the S-4800 Scanning Electron Microscope (SEM). It is for students, researchers, and first-time users.
Key points covered include:
1. Cleanroom Preparation to Prepare for and ALWAYS follow:
• Wear a lab coat, gloves, shoe covers, and eye protection (all provided in the lab).
• Follow cleanroom behavior protocols to maintain a contaminant-free environment.
• Always leave the microscopy laboratory in a nicer (cleaner) state than it was when you arrived.
2. Entering the Cleanroom
• Properly use and dispose of shoe covers.
• Move through clean zones to prevent particle contamination.
• If you have very messy shoes, leave them outside.
3. Filling the Cold Trap with Liquid Nitrogen
• Demonstration of correct filling procedure.
• Listen for the change in sound to know when it’s full—avoid overfilling. About 0.5 L is needed.
• Safety first: don’t pour liquid nitrogen on the machine or on yourself.
• Always use eye protection when working with liquid nitrogen.
4. Starting the SEM System
• Power is always on for the 4800 SEM, and check system status (e.g., idle mode).
• No need to start the microscope.
5. Using the Internal Camera
• Use the built-in camera for accurate sample positioning.
• Move slowly and carefully to avoid mistakes and dropping your sample inside the vacuum chamber.
6. Handling and Inserting Samples
• Clean sample handling techniques.
• Insert samples gently to protect the instrument and sample.
• More information on this in the next video.
7. Filling Out the Logbook
• Log your session start time, user name, and any relevant notes.
• Logging is mandatory: it helps staff track system use and plan maintenance.
• The SEM is not considered “in use” unless it’s properly logged.
• The SEM is booked (reserved via the LIMS system).
#sem #microscope #imaging
8. Why It Matters
• Ensures high-quality SEM imaging.
• Extends instrument lifetime and reduces repair costs
