Uploaded November 2025 | Updated September 2026, 38 minutes ago
*Enhance antibody development workflows by capturing full-length, paired variable regions with HiFi sequencing.* This webinar demonstrates how to identify high-affinity clones that can be missed by traditional screening methods.
Dr. Fortunato Ferrara from Specifica explains how overcoming the read-length limitations of short-read technologies enables the accurate characterization of single-chain variable fragments (scFv). Explore how this approach validates phage display libraries and powers AI model training with high-quality, paired-chain data.
*What you’ll learn*
* Sequence full-length, paired VL and VH chains to resolve antibody complexity.
* Overcome the low throughput of Sanger and the read-length limits of short-read sequencing.
* Identify rare, high-affinity binders that do not correlate with clone abundance.
* Generate high-quality training datasets for AI and machine learning models.
*Featured speakers*
* Nadia Sellami, PhD, Segment Lead, Biopharma (PacBio)
* Fortunato Ferrara, PhD, VP of Discovery (Specifica, an IQVIA business)
00:00 Introduction
02:50 What is HiFi sequencing?
11:23 Phage display workflow
13:40 Specifica’s antibody discovery platform
14:50 Overcoming short-read limitations
20:15 Library QC and validation
26:50 Expanding hits beyond Sanger
31:00 Does frequency equal affinity?
37:40 Generating data for AI training
46:20 AI antibody competition results
51:40 High-throughput Kinnex sequencing
54:37 Q&A
*Resources*
PacBio genomics: pacb.com
HiFi sequencing: pacb.com/technology/hifi-sequencing
How does full-length sequencing change your approach to library validation? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#AntibodyDiscovery #PhageDisplay #HiFiSequencing
*Enhance antibody development workflows by capturing full-length, paired variable regions with HiFi sequencing.* This webinar demonstrates how to identify high-affinity clones that can be missed by traditional screening methods.
Dr. Fortunato Ferrara from Specifica explains how overcoming the read-length limitations of short-read technologies enables the accurate characterization of single-chain variable fragments (scFv). Explore how this approach validates phage display libraries and powers AI model training with high-quality, paired-chain data.
*What you’ll learn*
* Sequence full-length, paired VL and VH chains to resolve antibody complexity.
* Overcome the low throughput of Sanger and the read-length limits of short-read sequencing.
* Identify rare, high-affinity binders that do not correlate with clone abundance.
* Generate high-quality training datasets for AI and machine learning models.
*Featured speakers*
* Nadia Sellami, PhD, Segment Lead, Biopharma (PacBio)
* Fortunato Ferrara, PhD, VP of Discovery (Specifica, an IQVIA business)
00:00 Introduction
02:50 What is HiFi sequencing?
11:23 Phage display workflow
13:40 Specifica’s antibody discovery platform
14:50 Overcoming short-read limitations
20:15 Library QC and validation
26:50 Expanding hits beyond Sanger
31:00 Does frequency equal affinity?
37:40 Generating data for AI training
46:20 AI antibody competition results
51:40 High-throughput Kinnex sequencing
54:37 Q&A
*Resources*
PacBio genomics: pacb.com
HiFi sequencing: pacb.com/technology/hifi-sequencing
How does full-length sequencing change your approach to library validation? *Comment below.*
*Subscribe for more PacBio genomics content:*
youtube.com/@PacificBiosciences?sub_confirmation=1
Learn more about PacBio at pacb.com
Legal & Trademarks: pacb.com/legal-and-trademarks
#AntibodyDiscovery #PhageDisplay #HiFiSequencing
