Sci- InspiSped up microscopic footage of oxygen bubbles in water produced from photosynthesis. I used the aquatic plant anacharis (Egeria densa) and a Marimo ball (Aegagropila linnaei).
Camera - Nikon D3300 Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
Photosynthesis Under the MicroscopeSci- Inspi2020-07-28 | Sped up microscopic footage of oxygen bubbles in water produced from photosynthesis. I used the aquatic plant anacharis (Egeria densa) and a Marimo ball (Aegagropila linnaei).
Camera - Nikon D3300 Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
1. Robert Brown F.R.S. Hon. M.R.S.E. & R.I. Acad. V.P.L.S. (1828) XXVII. A brief account of microscopical observations made in the months of June, July and August 1827, on the particles contained in the pollen of plants; and on the general existence of active molecules in organic and inorganic bodies , Philosophical Magazine Series 2, 4:21, 161-173, DOI: 10.1080/1478644280867476
2. Einstein, Albert (1905). "Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen" [On the Movement of Small Particles Suspended in Stationary Liquids Required by the Molecular-Kinetic Theory of Heat] (PDF). Annalen der Physik (in German). 322 (8): 549–560. Bibcode:1905AnP...322..549E. doi:10.1002/andp.19053220806How I Use Science To Capture Amazing FootageSci- Inspi2023-01-27 | Examples of how I use science to get great footage.
Camera - Nikon D3300
Microscope magnification of each shot is shown in the bottom right hand corner.
1. Shuo YAN, Wan-xing WANG, Jie SHEN, Reproductive polyphenism and its advantages in aphids: Switching between sexual and asexual reproduction, Journal of Integrative Agriculture, Volume 19, Issue 6, 2020, Pages 1447-1457, ISSN 2095-3119, doi.org/10.1016/S2095-3119(19)62767-X.
2. Kindlmann, P., and A. F. G. Dixon. “Developmental Constraints in the Evolution of Reproductive Strategies: Telescoping of Generations in Parthenogenetic Aphids.” Functional Ecology, vol. 3, no. 5, 1989, pp. 531–37. JSTOR, doi.org/10.2307/2389567. Accessed 9 Nov. 2022
3. Braendle, C., Davis, G., Brisson, J. et al. Wing dimorphism in aphids. Heredity 97, 192–199 (2006). doi.org/10.1038/sj.hdy.6800863
4. NG, J.C.K. and PERRY, K.L. (2004), Transmission of plant viruses by aphid vectors. Molecular Plant Pathology, 5: 505-511. doi.org/10.1111/j.1364-3703.2004.00240.x
5. almanac.com/pest/aphidsPoop Under The MicroscopeSci- Inspi2022-08-09 | *Typo at 1:01, the correct spelling is stercobilin, not stercoblin.
Microscopic video footage of poop under the microscope.
Cultures were grown on tryptic soy agar at 37 degrees Celsius for 24 hours.
Camera - Nikon D3300 Microscopes - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
1. Rose C, Parker A, Jefferson B, Cartmell E. The Characterization of Feces and Urine: A Review of the Literature to Inform Advanced Treatment Technology. Crit Rev Environ Sci Technol. 2015 Sep 2;45(17):1827-1879. doi: 10.1080/10643389.2014.1000761. PMID: 26246784; PMCID: PMC4500995.
2. Gray, Charles H. and Denis C. Nicholson. “627. The chemistry of the bile pigments. The structures of stercobilin and d-urobilin.” Journal of The Chemical Society (resumed) (1958): 3085-3099.
4. Reddy S, Sanders TA, Owen RW, Thompson MH. Faecal pH, bile acid and sterol concentrations in premenopausal Indian and white vegetarians compared with white omnivores. Br J Nutr. 1998 Jun;79(6):495-500. doi: 10.1079/bjn19980087. PMID: 9771336.
5. Triantafyllou K, Chang C, Pimentel M. Methanogens, methane and gastrointestinal motility. J Neurogastroenterol Motil. 2014 Jan;20(1):31-40. doi: 10.5056/jnm.2014.20.1.31. Epub 2013 Dec 30. PMID: 24466443; PMCID: PMC3895606.
6. S. Faramawy, T. Zaki, A.A.-E. Sakr, Natural gas origin, composition, and processing: A review, Journal of Natural Gas Science and Engineering, Volume 34, 2016, Pages 34-54, ISSN 1875-5100, doi.org/10.1016/j.jngse.2016.06.030.
7. Durack J, Lynch SV. The gut microbiome: Relationships with disease and opportunities for therapy. J Exp Med. 2019 Jan 7;216(1):20-40. doi: 10.1084/jem.20180448. Epub 2018 Oct 15. PMID: 30322864; PMCID: PMC6314516.
8. Durack J, Lynch SV. The gut microbiome: Relationships with disease and opportunities for therapy. J Exp Med. 2019 Jan 7;216(1):20-40. doi: 10.1084/jem.20180448. Epub 2018 Oct 15. PMID: 30322864; PMCID: PMC6314516.
11. Jiunn-Wei Wang, Chao-Hung Kuo, Fu-Chen Kuo, Yao-Kuang Wang, Wen-Hung Hsu, Fang-Jung Yu, Huang-Ming Hu, Ping-I. Hsu, Jaw-Yuan Wang, Deng-Chyang Wu, Fecal microbiota transplantation: Review and update, Journal of the Formosan Medical Association, Volume 118, Supplement 1,2019, Pages S23-S31, ISSN 0929-6646, doi.org/10.1016/j.jfma.2018.08.011.Dirty N95 Mask under the MicroscopeSci- Inspi2022-06-09 | Microscopic video footage of a clean and a dirty N95 mask under the microscope.
Cultures were grown on tryptic soy blood agar at 36 degrees Celsius for 48 hours.
1. Larsen, Gregory S., Cheng, Yongqiang, Daemen, Luke L., Lamichhane, Tej N., Hensley, Dale K., Hong, Kunlun, Meyer, Harry M., Monaco, Steven J., Levine, Alan M., Lee, Richard J., Betters, Emma, Sitzlar, Kim, Heineman, Jesse, West, Justin, Lloyd, Peter, Kunc, Vlastimil, Love, Lonnie, Theodore, Merlin, and Paranthaman, Mariappan Parans. Polymer, Additives, and Processing Effects on N95 Filter Performance. United States: N. p., 2021. Web. doi:10.1021/acsapm.0c01294.
2. Adanur S, Jayswal A. Filtration mechanisms and manufacturing methods of face masks: An overview. Journal of Industrial Textiles. December 2020. doi:10.1177/1528083720980169
3. Taslim Ur Rashid, Sadia Sharmeen, and Shanta Biswas. Effectiveness of N95 Masks against SARS-CoV-2: Performance Efficiency, Concerns, and Future Directions. ACS Chemical Health & Safety 2022 29 (2), 135-164 DOI: 10.1021/acs.chas.1c00016
4. William G. Lindsley, Francoise M. Blachere, Brandon F. Law, Donald H. Beezhold & John D. Noti (2021) Efficacy of face masks, neck gaiters and face shields for reducing the expulsion of simulated cough-generated aerosols, Aerosol Science and Technology, 55:4, 449-457, DOI: 10.1080/02786826.2020.1862409
5.Leggett, Helen C et al. “Mechanisms of pathogenesis, infective dose and virulence in human parasites.” PLoS pathogens vol. 8,2 (2012): e1002512. doi:10.1371/journal.ppat.1002512
6.Brooks JT, Butler JC. Effectiveness of Mask Wearing to Control Community Spread of SARS-CoV-2. JAMA. 2021;325(10):998–999. doi:10.1001/jama.2021.1505
7.Yim, Wonjun et al. “KN95 and N95 Respirators Retain Filtration Efficiency despite a Loss of Dipole Charge during Decontamination.” ACS applied materials & interfaces vol. 12,49 (2020): 54473-54480. doi:10.1021/acsami.0c17333Ice Melting Under the MicroscopeSci- Inspi2022-01-06 | Microscopic video footage of ice melting in real time.
Camera - Nikon D3300 Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
An experiment testing water purification tablets on lake water, and examining the water through a microscope and a petri dish. The tablets are only effective against microbes and not heavy metals and chemicals.
The medium used for the petri dish was tryptic soy agar. The dish was incubated for 24 hours at 37 degrees Celsius.
Camera - Nikon D3300 Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
An experiment testing water purification tablets on dirty water, and examining the water through a microscope and a petri dish. The tablets are only effective against microbes and not heavy metals and chemicals.
The medium used for the petri dish was tryptic soy agar. The dish was incubated for 24 hours at 37 degrees Celsius.
Camera - Nikon D3300 Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
Media used in the previous video is tryptic soy agar (TSA). TSA can grew some acetic acid bacteria, but the bacteria in the vinegar didn't. I assumed that meant they were dead, but this shows I was wrong and they are alive.
Camera - Nikon D3300 Microscopes - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
The milk was collected through hand expression after the mother washed her hands with soap and water and was place in a cooler with ice. About an hour later it was plated on a petri dish and observed under the microscope.
I used a tryptic soy agar petri dish to grow the microbes in the breast milk. It was incubated for 24 hours at 37 degrees Celsius.
Camera - Nikon D3300
Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
At the end of the video I used tryptic soy agar petri dishes to grow the microbes in the dirty water before and after it was filtered. It was incubated for 24 hours at 37 degrees Celsius.
Camera - Nikon D3300
Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
I deduced I had Group-C streptococcus laryngitis based on the following criteria:
*Contact with someone who had a strep infection *Sore throat and difficulty speaking, infection lasted about a week *I had gray spots on my tongue although none on my tonsils *Gram positive streptococcus isolated from the white spots on my tongue *Streptococcus was beta-hemolytic and resistant to bacitracin and Sulfamethoxazole / Trimethoprim
My education is not in medicine so I could be wrong.
Camera - Nikon D3300 Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
Spotify: open.spotify.com/artist/2D9Q2aAhQb6dh3aLmZaHWaCopper Sulfate Crystals Forming under the MicroscopeSci- Inspi2020-01-20 | Microscopic footage of copper sulfate crystals forming under the microscope. The first few shots are in real time and the rest are sped up.
Copper sulfate is used as a fungicide and herbicide as well as a reagent in laboratories.
Camera - Nikon D3300
Microscopes- Wolfe Laboratory Steromicroscope
Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
Spotify: open.spotify.com/artist/2D9Q2aAhQb6dh3aLmZaHWaKimchi under the microscopeSci- Inspi2019-12-18 | Footage of kimchi as seen under the microscope. Kimchi is fermented cabbage, also known as Baechu kimchi. Many vegetables can be fermented to make kimchi, although cabbage kimchi is the most recognized worldwide.
Typo correction: at 0:48, it should be "breathe" not "breath".
I used two batches of kimchi. One was canned and the other was from a refrigerated jar. The canned kimchi had less microbes and allowed for the oil droplets to be more easily seen.
At the end of the video I used a tryptic soy agar petri dish to grow the microbes in kimchi. It was incubated for 24 hours at 37 degrees Celsius.
Camera - Nikon D3300
Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
At the end of the video I used a tryptic soy agar petri dish to grow the microbes in kombucha. It was first incubated for 24 hours at 37 degrees Celsius which had some growth of tiny colonies. I incubated it for 48 additional hours at room temp to allow these colonies more time to grow.
Camera - Nikon D3300
Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
I then add simulated stomach acid to see how the acid will change the pH. I created the simulated stomach acid by making a 3.16 mM hydrochloric acid solution to mimic average fasting stomach acid with a pH of 2.5 (Range 1.5-3.5). I also added 5 grams per liter of sodium chloride and 5 grams per liter of potassium chloride to better mimic stomach acid.
Music by Sci-inspi.Diatomaceous Earth under the microscopeSci- Inspi2019-10-17 | Microscopic footage of Diatomaceous Earth as seen under the microscope.
For more great footage and info on diatoms, check out Journey to the Microcosmos' video on diatoms: youtube.com/watch?v=Ygty9HxhFK4
Camera - Nikon D3300
Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
Spotify: open.spotify.com/artist/2D9Q2aAhQb6dh3aLmZaHWaChaos carolinense under the microscopeSci- Inspi2019-09-27 | Microscopic footage of Chaos carolinense ( also known as Pelomyxa carolinensis) as seen under the microscope. C. carolinense moves by amoebiod movement which is a crawling-like motion. They can be found in many freshwater environments like rivers, lakes and ponds.
Camera - Nikon D3300
Microscope - Leica ATC 2000
Microscope magnification of each shot is shown in the bottom right hand corner.
The petri dish was incubated for 24 hours at 37 degrees Celsius.
Recorded with a Nikon D3300 DSLR camera. The microscope used was a Leica ATC 2000
Music by Sci-inspiToxic House Mold Under the MicroscopeSci- Inspi2019-07-06 | Microscopic footage of toxic house mold. Two fungi can be seen here: Chaetomium and either Penicillium or Aspergillius. . Mold grows in damp environments, so the best way to prevent mold growth is to keep your house dry and fix any leaks you find. Mold can be dangerous as they produce myctoxins, which are toxins that can affect human health.
A Leica ATC 2000 Microscope was used to view the mold. Captured with a Nikon D3300
The microscope magnification of each shot is shown in the bottom right hand corner.
Music created by Sci-inspi.What does it take to kill a bacterial spore? Petri dish timelapseSci- Inspi2019-06-16 | An experiment showing different environmental conditions bacterial spores can survive through. Petri dishes were incubated for 24 hours at 37 degrees Celsius. Each time-lapse shot is 24 hours long.
Drums by David Maya Guitar by Sci-inspiC. elegans under the MicroscopeSci- Inspi2019-05-14 | Microscopic footage of the non-parasitic nematode worm C. elegans. C. elegans is studied by scientist all over the world in order to learn more about sleep, genetics, metabolism and more.
The microscope magnification of each shot is shown in the bottom right hand corner.
Music created by Sci-inspi.Expired Milk Under the MicroscopeSci- Inspi2019-04-07 | Microscopic footage of milk before it expires, after it expires on its "best-by-date" and after being incubated to speed up microbial growth. I also tested for bacteria at each stage with a petri dish to quantify bacterial colonies.
I believe the most common bacteria after the milk was incubated to be lactobacillus because of the following criteria: gram positive rods, growth on complex medium (milk, Tryptic soy agar), non-pathogenic since the milk was pasteurized, Indole negative, Citrate negative. This is the best guess I can make with the medium and tests available to me in my lab.
Q: The milk was pasteurized. Where did the microbes come from?
A: Pasteurization kills most but not all microbes. The ones that survived grow in the milk.
I used a Nikon D3300 DSLR camera and a Leica ATC 2000 Microscope for this video.
The Microscope magnification of each shot is shown in the bottom right hand corner.
Music is written and produced by Sci-inspi.Salt Crystals Forming under the MicroscopeSci- Inspi2019-03-10 | Time-lapse footage of salt crystals forming under the microscope.
Camera - Nikon D3300
Microscope - Leica ATC 2000Sci-inspi Music Out Now!Sci- Inspi2019-03-02 | 27 songs are now out to buy or stream online. Get my music on:
Thank you everyone who has supported me and my channel.
More to come!White Blood Cells under the MicroscopeSci- Inspi2019-02-18 | Microscopic footage of different white blood cell types found in blood. Some of this footage is sped up to show how phagocytes move around and look for pathogens. This crawling motion is called amoeboid movement and it is used by many different eukaryotes.
Camera - Nikon D3300
Microscopes -
Leica ATC 2000 - 40X, 100X, 400X, 1000X American Optical One - Ten microscope - 150X, 600X, 1500X
The microscope magnification of each shot is shown in the bottom right hand corner. Footage that has been sped up is indicated so at the bottom of the shot.Spore Stain Procedure (Schaeffer-Fulton Method)Sci- Inspi2019-02-05 | Bacterial Endospore stain procedure. This protocol is taken from "Benson's Microbiological Applications: Laboratory Manual in General Microbiology" Eleventh Edition
1. Cover the bacteria smear with a piece of paper towel and saturate it with malachite green. Steam it over boiling water for 5 minutes and add more stain as it boils off.
2. Allow the slide to cool, remove the piece of paper towel and rinse for 30 seconds.
3. Counterstain with safranin for about 20 seconds.
4. Rinse briefly with water to remove safranin
5. Blot dry and view under the microscope in immersion oil.
Camera: Nikon D3300
Microscopes: Leica ATC 2000 - 1000X American Optical One - Ten microscope - 1500XBoiled Dirty Water under the MicroscopeSci- Inspi2019-01-23 | A microscopic examination of dirty water before boiling and after boiling.
Q: Should the plant material be removed before boiling?
A: Yes. To make water potable it should be filtered and boiled. In this video I wanted to show the antimicrobial properties of boiling even with the plant material in the beaker.
Q: Is the water safe to drink after boiling?
A: Boiling kills bacteria, but it does not get rid of any toxins, heavy metals or bacterial spores. It should be filtered or distilled to get rid of those items.
The microscope magnification in each shot is shown in the bottom right hand corner.
Camera - Nikon D3300
Microscope - Leica ATC 2000Crickets under the MicroscopeSci- Inspi2018-12-27 | House crickets, Acheta domesticus and Gryllodes sigillatus, as seen under a microscope.