Leveraging isoform-level RNA sequencing to understand rare disease pathogenesis. PacBio 2026-09-21 | Leveraging isoform-level RNA sequencing to understand rare disease pathogenesis.
Increasing the throughput of full-length 16S and RNA-Seq with Kinnex™ kits PacBio 2024-01-05 | Explore the transformative capabilities of Kinnex kits from PacBio, in reshaping the landscape of microbiome community profiling and RNA-Seq in plant and animal research. This webinar guides you through the enhanced functionalities of full-length 16S rRNA and RNA-Seq, empowered by the new Kinnex kits (using MAS-Seq technology/method), which facilitate a substantial increase in the number of reads obtained from PacBio long-read sequencing platforms. Delve into detailed customer scientific studies that showcase the practical applications and influential impact of Kinnex kits in contemporary research environments. Learn more about PacBio at pacb.com Legal & Trademarks: Visit pacb.com/legal-and-trademarks
Read Segmentation and Iso-Seq workflow in SMRT Link PacBio 2023-12-13 | Demo video for Read Segmentation and Iso-Seq workflow in SMRT Link v13.0 and up. The workflow supports full-length isoform analysis for data generated on PacBio using Kinnex full-length RNA kit. The end-to-end workflow begins with HiFi reads and outputs full-length isoform classifications with supporting read count information.Learn more about Kinnex at: pacb.com/kinnexLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Sample Setup and Run Design for Kinnex kits in SMRT Link PacBio 2023-12-13 | Demo video for Sample Setup and Run Design for Kinnex kits in SMRT Link v13.0 and up. The Kinnex kits support full-length RNA sequencing (Kinnex full-length RNA kit), full-length 16S rRNA sequencing (Kinnex 16S rRNA kit) and full-length single-cell RNA sequencing (Kinnex single-cell RNA kit). Learn more about Kinnex at: pacb.com/kinnexLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Analyzing Kinnex 16S rRNA data in SMRT Link PacBio 2023-12-13 | Demo video for analyzing Kinnex 16S rRNA data generated using Kinnex 16S rRNA kit in SMRT Link v13.0 and up. Learn more about Kinnex at: pacb.com/kinnexLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Welcome to the accuracy era — sequencing with Onso and SBB PacBio 2023-12-01 | In this video, we delve into the cutting-edge world of Q40+ accuracy with the PacBio Onso System, showcasing the remarkable benefits of our proprietary chemistry, Sequencing by Binding (SBB).Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Advancing Cardiology Research with Long-read RNA Sequencing PacBio 2023-11-29 | In this webinar, learn how long-read transcriptomics at full-length isoform resolution is transforming our understanding of heart development, function, and disease. Two researchers discuss their use of bulk and single-cell sequencing with the PacBio Iso-Seq method for cardiology research, which includes a comparison to Oxford Nanopore and short-read RNA sequencing, discuss applications in spatial transcriptomics, and review early data generated from the recently released Kinnex full-length RNA kit.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Expanding NGS truth sets: more comprehensive variant detection in a multi-generational pedigree PacBio 2023-11-21 | PacBio CoLab #2 part 2 at ASHG 2023Expanding NGS truth sets with long-reads: more comprehensive variant detection in a multi-generational pedigree.Accurate long-read sequencing characterizes the full spectrum of genetic variation across the genome, but variant calling software is still catching up to the sequencing technologies. We have generated deep Pacific Biosciences (PacBio) high-fidelity (HiFi), ultra-long Oxford Nanopore Technologies (ONT), Strand-seq, and Illumina whole-genome sequencing data to construct near-T2T, phased genome assemblies from primary material obtained from a 4-generation, 28-member CEPH pedigree (1463). We are constructing a more comprehensive and validated catalog of greater than 8 million single-nucleotide variants, indels, short tandem repeats, and structural variants, including a detailed assessment of inversion polymorphisms that associate with disease risk. The use of multiple orthogonal technologies, near-T2T phased-genome assemblies, and a multi-generation family allow us to assess inheritance patterns and to create a “truth set” for all classes of human genetic variation upon which to test and benchmark new technologies.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Breakthrough discoveries in genomics, transcriptomics and epigenomics with HiFi long-read sequencing PacBio 2023-11-21 | 0:00 - Christian Henry introduction1:29 - Jonas Korlach - Breakthrough discoveries in genomics, transcriptomics and epigenomics with HiFi long-read sequencing12:25 - Andrew Stergachis - Multi-ome long-read sequencing for resolving Mendelian conditions33:04 - Gloria Sheynkman - From genome to transcriptome to protein functions - uncovering new biology and clinical applicationPacBio ASHG 2023 WorkshopThis workshop delves into the forefront of genomics research! Discover how innovative researchers are leveraging the extraordinary accuracy and comprehensive coverage of HiFi sequencing to drive groundbreaking insights into the intricate world of genetic variation, RNA splicing, and epigenetic gene regulation.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Revio long-read sequencing: Looking back on year one PacBio 2023-11-21 | Dive into the remarkable journey of the Revio long-read sequencer since its announcement at ASHG in October of 2023. This video is a celebration of milestones and an exploration of how Revio is reshaping the landscape of genomic sequencing.Over the past year, the Revio sequencer has been installed in over 125 labs worldwide, marking a significant leap in HiFi sequencing capabilities. It has outperformed its predecessors, like the Sequel II, in terms of yield and efficiency, and what this means for the future of genomic research.Our journey includes firsthand accounts from scientists and researchers who have witnessed the transformative impact of Revio in their work. From drastically reducing sequencing queues to achieving unprecedented HiFi data outputs, these testimonials highlight the real-world implications of this technological advancement.But what does this all mean for the field of genomics and beyond? Revio enhances our understanding of complete genomes, genomic phasing, native epigenetics, and the analysis of repetitive regions.As we look to the future, we emphasize the potential of the Revio sequencer in driving forward human health research. This video is a call to action for researchers and enthusiasts alike to be a part of this exciting journey.Join us as we celebrate the achievements of the past year and look forward to the possibilities that Revio brings to tomorrow's genomic research.Don't forget to subscribe for more updates and insights into the world of genomics and technological innovation in science.Learn more about the Revio system here pacb.com/revioLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Exploring the potential clinical utility of HiFi sequencing for homologous loci (pseudogenes) PacBio 2023-11-21 | 0:00 -Alex Robertson - HiFi Solves, a global consortium of clinical genomics researchers (brief introduction and updates)2:45 - Alexander Hoischen - Exploring the potential clinical utility of HiFisequencing for homologous loci (pseudogenes)PacBio HiFi long-read sequencing has the potential to become a single front-line assay for interrogating rare disease cohorts because of its ability to accurately call and phase all classes of variants. An international group of clinical researchers and PacBio have been collaborating to study the efficacy of HiFi sequencing in interrogating the many possible genetic mechanisms that underlie rare diseases. This presentation provides an overview of the group’s goals and activities and recent results, particularly around correctly resolving clinically relevant genes that lie in areas of high sequence homology or otherwise complex regions of the human genome.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
A new age in liquid biopsy research with highly accurate short-read sequencing. PacBio 2023-10-31 | In this webinar, learn about game-changing liquid biopsy research capabilities made possible by the Onso sequencing system and powered by proprietary sequencing by binding (SBB) chemistry.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Changing the game with Revio – Pamela Nicholson PacBio 2023-10-10 | Turning Revios into reality… Proud new Revio owner Pamela Nicholson, PhD, Platform Manager at the University of Bern finds the high throughput of a Revio system ‘really impressive’ and a game changer for researchers who want to scale their HiFi projects on academic budgets.Unlock new insights faster with one complete, phased 30x human genome for each Revio SMRT Cell. Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Achieving more comprehensive variant detection at scale in rare disease research PacBio 2023-10-05 | Higher throughput and lower costs for highly accurate long-read (HiFi) sequencing are now available on the Revio system. HiFi sequencing enables you to capture all variant types with high precision and recall, phase haplotypes, and identify methylation patterns across the genome.Dr. Adam Ameur of SciLifeLab in Uppsala, Sweden, has implemented the Revio system to create new discovery opportunities for a range of HiFi sequencing applications, including whole-genome sequencing in human genomic research.In this webinar Dr. Ameur discusses:• More comprehensive detection of SNVs, SVs, tandem repeats, and epigenetic signals. • Near-complete diploid assemblies generated to find complex, potential disease-causing SV regions. • Use of the Revio system in future population-scale and single-cell sequencing research.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Understanding SMRT Link run QC and on-instrument analysis on the PacBio Revio system PacBio 2023-10-05 | In this webinar, PacBio scientists Nancy Francoeur, PhD and Caroline Storer, PhD will help you understand Revio performance metrics and on-instrument analysis options to put you in control of your HiFi sequencing runs. Primary run QC metrics in SMRT Link let you evaluate sequencing performance, monitor performance trends, and understand how sample loading affects HiFi sequencing quality and yield. Watch this webinar to find out more about: HiFi sequencing primary run QC metrics and terminology. Interpretation of key metrics and visualizations to evaluate sequencing performance. Revio system on-instrument analysis pipelines, output files, and folder structure. Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
A pangenome reference of Chinese populations PacBio 2023-10-03 | Human genomics is witnessing an ongoing paradigm shift from a single reference sequence to a pangenome form, yet populations of Asian ancestry remain underrepresented. In Dr. Shuhua Xu's latest Nature article 'A Pangenome Reference of 36 Chinese Populations', the Chinese Pangenome Consortium (CPC) attempted to uncover missing sequences and previously unidentified hidden variations within Chinese ethnic groups. CPC also aimed to establish a high-quality genome reference for comprehensive profiling gene diversity in Chinese as well as other Asian populations.In this webinar, Dr. Shuhua Xu, a professor of human population genetics and the Principal Investigator of the Population Omics Group at Fudan University, introduces his team's new findings in their latest Nature article.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Library preparation and HiFi sequencing applications for AAV PacBio 2023-09-27 | In this webinar, Edward Oakeley, PhD, Novartis, shares and explains his experience and the developments of the HiFi AAV genome sequencing method over time and how his group has developed a modified library preparation protocol to maximize the usefulness of PacBio HiFi reads for AAV characterization. The modified library prep protocol includes adding an additional step for the synthesis of a complementary second strand in order to capture a greater variety of AAV molecules in the analysis. Gain a deep understanding of the unique advantages that HiFi sequencing brings to the table, empowering you to make informed decisions that can significantly impact the safety and efficacy of gene therapy products.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Long-read RNA neurology symposium PacBio 2023-09-22 | 0:00 – Intro3:08 – High-throughput full-length isoform sequencing for bulk & single-cell transcriptome24:15 – Moving towards a resolved atlas of cell type isoforms in the human cortex43:16 – Long-read RNA-seq of human microglia reveals novel isoforms and elucidates genetic regulation of splicing associated with Alzheimer's and Parkinson's disease58:38 – Cell-type-specificity of isoform diversity in the developing human neocortex informs mechanisms of neurodevelopmental disorders1:28:50 – Visualizing Iso-Seq data with Shiny app1:44:16 – Single-cell isoform sequencing in neurodegenerative disease2:00:00 – Unravelling the role of transcript expression in neurodegenerative disease: insights from long-read RNA sequencing2:23:15 – Speaker panel discussionIn this virtual symposium, hear from a variety of researchers who are using the PacBio Iso-Seq method for bulk and single-cell transcriptomics to drive cutting-edge research in neurology, neurodegenerative diseases, and brain development. Learn about the MAS-Seq for 10x Single Cell 3’ kit and get a sneak preview of the upcoming MAS-Seq for bulk Iso-Seq kit. Discover how long-read HiFi sequencing provides extraordinary discovery power for uncovering new insights in RNA biology.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Customer testimonial – TGen explores the next-level accuracy of Onso short-read sequencing. PacBio 2023-09-13 | Experience a new era of short-read sequencing accuracy with the Onso system. In this illuminating video, Candice Wike, Ph.D., from the Translational Genomics Research Institute (TGen), part of City of Hope, joins Product Marketer Young Kim in Menlo Park. During their conversation they take us on a journey through the remarkable world of TGen’s research and highlights the game-changing potential of this advanced technology. TGen, located in Phoenix, AZ, is a nonprofit biomedical research institute focused on precision medicine in areas such as oncology, pathogens and infectious diseases, rare childhood disorders, and neurological disorders.In discussing her experience with PacBio’s Onso system, Dr. Wike highlights the system's exceptional accuracy, spotlighting its capacity to detect ultra-low variants with unprecedented precision. The significance of this accuracy is emphasized as she discusses the beauty of mapped reads and the statistical power they offer, enabling the detection of variants at an astonishingly low frequency of 0.001%. She also explains how the high accuracy from the technology’s sequencing by binding (SBB) chemistry, reduces the need for complex library prep methods and intricate downstream bioinformatics pipelines in the short-read sequencing space. With the PacBio Onso system, researchers like Dr. Wike can trust that the data they see is a direct representation of their findings, removing the need for additional error correction processes.Beyond the technical intricacies, Dr. Wike discusses PacBio’s collaboration with TGen and how Onso, with its ability to detect variants at such high accuracy, will enable TGen to continue asking the questions that push the boundaries of precision medicine.Learn more about SBB chemistry at pacb.com/technology/SBBLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Changing the game with Revio – Arthur Dondi PacBio 2023-09-13 | Hear Arthur Dondi, a PhD candidate from ETH Zürich discuss how he envisions Revio's higher throughput and accuracy will advance his research in ovarian cancer therapy.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Iso-Seq Social Club, Vol. 3 - Bulk Iso-Seq applications: from disease to function PacBio 2023-09-05 | This video contains the following talks:Isoform resolved transcriptome of the human preimplantation embryo - Denis TorreUnderstanding the boundaries between PTSD and depression through alternative splicing - Sydney Muchnik, PhDNovel approaches for studying RBP-RNA interactions at isoform-level resolution - Pratibha JagannathaCharacterization of protein isoform diversity in human umbilical vein endothelial cells (HUVECs) via long-read proteogenomics - Madison Mehlferber
Iso-Seq Social Club, Vol. 3 - TappAS for isoform differential expression analysis PacBio 2023-09-05 | This video is a practical long-read sequencing tutorial, covering the basics of functional iso-transcriptomics using TappAS and how to use TappAS for analysis, presented by Angeles Arzalluz-Luque, PhD Candidate from the Conesa Lab in Valencia Spain.
Iso-Seq Social Club, Vol. 3 - Introduction to Iso-Seq method PacBio 2023-09-05 | In this video, Liz Tseng, PhD, Associate Director for PacBio and specialist in the Iso-Seq method will explain what the method entails and how single-cell isoform sequencing can change the game for the omics.
Iso-Seq Social Club, Vol. 3 - SQANTI3 for isoform classification, annotation and curation PacBio 2023-09-05 | Watch this video to hear from PhD Candidate Francisco J. Pardo Palacios and Liz Tseng, PhD as they explain the three steps required to create a reference library for isoform transcription
Iso-Seq Social Club, Vol 3. - Bioinformatics tools for Iso-Seq and single-cell Iso-Seq analysis PacBio 2023-09-05 | In this video presentation, learn about the single-cell Iso-Seq workflow and join Roger Volden, PhD for a SMRTLink preview and command line walkthrough. Also, see Anoushka Joglekar, PhD talk about visualizing isoform expression across multiple conditions.
Iso-Seq Social Club, Vol. 3 - Single-cell Iso-Seq applications in cancer and neurological disorders PacBio 2023-09-05 | This video includes the following talks:Who needs more ACTB isoforms? Discover more from your single-cell Iso-Seq sample with CRISPR-Cas9 depletion - Jon ArmstrongWhen single-cell resolution isn’t enough: going beyond short reads with new approaches to uncover the roots of tumor heterogeneity - Antonio Fuentes-Fayos, PhDIntegrating single cell genotyping with transcriptomics to identify disease-involved brain cell types in pediatric epilepsy - Katie Miller, PhDSingle cells + single molecules = isoform biology - Jason Underwood, PhD
Optimizing and Confirming AAV Vector Design with PacBio Sequencing PacBio 2023-09-05 | This bioinformatics webinar explores how to generate and analyze full-length AAV genomic data with PacBio HiFi sequencing: a cutting-edge long read technology for profiling packaged genomes as a single intact molecule, measuring impurities, and verifying that vector capsids contain your intended design. The presenter is PacBio scientist Dr. Elizabeth Tseng and the guest panel consists of research leaders at Homology Medicines and AavantiBio who discuss how, when, and where they use the PacBio AAV sequencing to drive innovation in their gene therapy research programs.Read our latest Blog on full-length AAV sequencing: pacb.com/blog/hifi-sequencing-tools-to-help-transform-aav-based-gene-therapy Learn more about PacBio at pacb.com Legal & Trademarks: Visit pacb.com/legal-and-trademarks
Actionable genomics at scale | PacBio Workshop at PAG 2023 PacBio 2023-09-05 | 00:00 - 30:10 "Opening remarks"48:57 - 1:14:45 "Conserving California’s biodiversity with genomics"1:14:46 - 1:35:15 "Exploring the multiple evolutions of sex chromosomes in plants"1:35:16 - 1:54:48 "More and better: a new era for metagenome assembly" Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
The value of Q40+ sequencing accuracy with SBB for liquid biopsy PacBio 2023-08-29 | Liquid biopsy is revolutionizing cancer research through non-invasive detection of tumor DNA in the blood. However, sequencing errors introduced by traditional next-generation sequencing can mimic real mutations, obscuring the true signal. The PacBio Onso short-read sequencing system, powered by our highly accurate SBB technology, can reduce false positives and improve sensitivity for detecting rare variants in ctDNA, potentially enabling a new generation of liquid biopsy assays for more sensitive tumor DNA detection.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
A new era of short-read accuracy is here – SBB Q40+ accuracy on the Onso system PacBio 2023-08-29 | A new era of short-read sequencing accuracy is now available with the groundbreaking PacBio Onso system and its revolutionary sequencing by binding (SBB) chemistry.In this video, we embark on an exploration of our new short-read sequencer, diving into how it works from start to finish. See the process of converting various samples – whether high molecular weight, pre-fragmented, sheared, or PCR amplicons – into compatible library structures ready for seamless sequencing and explore how Onso library conversion kits can effortlessly transform your existing libraries into an Onso-compatible format. From library prep to clustering, the Onso short-read sequencing system’s workflow is easy-to-use. In this video, we demonstrate how our efficient design allows a single cluster generator to support multiple Onso systems, streamlining your sequencing journey.The heart of our short-read accuracy innovation lies in the proprietary chemistry that drives near-perfect accuracy. With SBB you can wave goodbye to molecular scarring and residual linker arms and say hello to reduced phasing and stuttering errors in order to embrace a new era of precision.The Onso system’s SBB chemistry stands out. Achieving ≥90% of bases at Q 40+, with minimal duplication rates, its remarkable ability to tackle intricate regions with ease is what sets it apart. On top of it all, data analysis is easier than ever on the Onso system. With industry-standard FASTQ file outputs, data seamlessly integrates into existing pipelines and helps facilitate secondary analysis. Onso sequencing even delivers the ability to onboard demultiplexing, generating FASTQ files for each pooled sample.Seize the opportunity to revolutionize you short-read sequencing journey with Onso’s SBB chemistry. Learn more about SBB chemistry at pacb.com/technology/SBBLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Changing the game with Revio – Prof Lars Feuk PacBio 2023-08-29 | Hear Lars Feuk, a professor of molecular medicine at Uppsala University, discuss how the Revio system will have a transformative impact on his research involving single nucleotide variations (SNVs).Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Changing the game with Revio – Prof Anna Lindstrand PacBio 2023-08-29 | Hear Anna Lindstrand, a professor in Clinical Genetics at the Karolinska Institute and director of the Clinical Genetic diagnostic laboratory at Karolinska University Hospital, discuss how Revio will be game changing in her rare disease research.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
The Full-Length Transcriptomic Atlas of Human Colorectal Cancer from Single-Cell Isoform Sequencing PacBio 2023-08-24 | In this webinar, Zhongxiao Li, share insights from his collaborative work at King Abdullah University of Science and Technology (KAUST) with Cancer Science Institute of Singapore (CSI), National University of Singapore (NUS). Together, they created the first-ever isoform-resolution single-cell transcriptomic atlas of colorectal cancer (CRC). Discover how they also leveraged PacBio HiFi sequencing to accurately identify recurring neoepitopes.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Keynote presentation - New biology from new genomes: fruits from the Tree of Life PacBio 2023-07-13 | Mark Blaxter leads the Wellcome Sanger Institute’s Tree of Life programme, which is using PacBio HiFi sequencing to generate and analyse high-quality reference genomes for many thousands of species across the tree of life. In this presentation, Mark discusses his team's research portfolio focusing on the genomics of neglected, non-model organisms and the interpretation of those genomes in ecological and evolutionary contexts (including, inter alia, parasitic and free-living nematodes, tardigrades, gastropod and bivalve molluscs, butterflies, bees, flies, birds, algae, fungi and bacteria). Watch his full Q&A session.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Streamlined chromosome-scale phasing with HiFi sequencing PacBio 2023-06-27 | Reconstructing complete phased genomes is key to understanding the genetic basis of many complex traits. However, the bioinformatic complexity of long-range phasing creates a pressing need for streamlined, efficient computational approaches that can reconstruct high-quality, chromosome-scale phased sequences at scale.Highly accurate long reads from PacBio (HiFi reads) are well suited for haplotype-resolved assemblies of human, non-human, and cancer genomes. With the right bioinformatics tools, HiFi data enables high-quality phased genome assembly to examine structural variant landscapes, characterize cancer-related mutations, and facilitate new, unbiased studies of haplotype variation.In this webinar, Shilpa Garg, PhD, associate professor at DTU Biosustain, discusses:• The need for chromosome-scale phasing in reference genomes • Efficient and accurate haplotype-resolved human genome assembly • Generalized graph-based methods for phased assembly of cancer genomesLearn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Shining a Light on Dark Genes in Population Sequencing. PacBio 2023-06-26 | During this exciting webinar, Fritz Sedlazeck, PhD from Baylor College of Medicine and Kiran Garimella, PhD from the Broad Institute and All of Us program explain how HiFi long-read sequencing is energizing the potential of population genomics by enabling scientists to catalogue all genetic variation, reflecting a population’s true diversity, and maximize discovery potential to enable the most accurate insights possible.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Detecting cancer-related RNA dysregulation with long-read sequencing PacBio 2023-05-15 | Full-length, single-cell RNA data provides critical insights into the understanding of cancer transcriptomic features, such as isoforms, fusions, and expressed mutations. However, until recently, cell typing based on gene expression has been challenging with long reads due to insufficient sequencing depth.In a recent preprint, Dondi et al. (2022) used transcript concatenation and highly accurate long-read sequencing (HiFi sequencing) to increase sequencing depth. This increase enabled cell type identification without needing short reads, and the detection of many new isoforms. They also detected fusions, cell-specific and cell type-specific isoform usage, and revealed differential isoform expression in tumor and mesothelial cells.The authors conclude that “future studies with similar or increased long-read throughput will not have to rely on parallel short-read sequencing, thereby saving cost and labor.” In this webinar Arthur Dondi discusses:• Detection of cell type-specific known and novel isoforms• Capture and quantification of full-length isoforms, mutations and fusions in the same scRNA-seq dataset• Detection of a gene fusion that was misclassified in matched short-read dataLearn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Understanding clonal evolution using game theory and single-cell long-read isoform analysis. PacBio 2023-04-17 | In this webinar, Dr. Nathan Salomonis of Cincinnati Children’s Hospital Medical Center presents how he used the MAS-Seq kit and sequencing on the PacBio Revio system to provide new insights into leukemia clonal evolution, through paired single-cell mutation, transcription, splicing, cell-surface, and cell state analysis. Learn how this methodology allowed for the characterization of unique lineages, an important step in single-cell transcriptomics and cancer research.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Jake Brandenburg - PacBio AGBT-Ag 2023 Workshop Talk PacBio 2023-04-05 | Long read sequencing at scale with PacBio's new Revio system. Come learn about ultra-high throughput, sample-to-answer solutions for agrigenomics built around the Revio system and HiFi reads from PacBio. This solution includes automated workflows for whole genome sequencing, complete with epigenetics, as well as high throughput genome annotation with our new MAS-seq kits for RNA solutions.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Descubra el poder de las secuencias largas y de alta precisión PacBio 2023-04-05 | Con este seminario web descubrirá como las secuencias largas y de alta precisión le ayudarán a lograr nuevos descubrimientos en sus investigaciones, y cómo el nuevo sistema Revio está revolucionado el mundo de la secuenciación genómica.Descubrimientos científicos avanzados requieren datos de secuenciación que sean precisos y completos. Las secuencias largas y de alta precisión (secuencias HiFi) proporcionan una combinación única de ambos que permiten a los científicos producir ensamblados de genomas de alta calidad, detectar transcritos completos, identificar sitios de metilación de ADN y determinar una amplia gama de tipos de variantes genómicos.Investigadores destacados han aplicado con éxito la tecnología de secuenciación de PacBio para resolver ensamblados de genomas completos. Además, las secuencias HiFi proporcionan un enfoque de mayor resolución para estudiar las variantes estructurales y su papel en la salud y las enfermedades humanas. Con Revio, nuestro último lanzamiento, nos permite ofrecerle un mayor rendimiento y un menor coste de secuenciación de datos de alta fidelidad, que a su vez benefician a un número cada vez mayor de áreas de investigación.In this webinar, find out how highly accurate long reads can enable you to forge a new frontier in genomics – and how the game-changing new Revio system can make it happen. Groundbreaking discoveries demand the best data possible. The length and accuracy (99.9%) of PacBio long-read (HiFi) sequencing allows you to generate fully phased reference-quality assemblies that include methylation status and the ability to see complex variants even in dark regions – all from a single sequencing run.Leading researchers have successfully applied PacBio HiFi sequencing to obtain fully phased de novo assemblies of even the most complex genomes. In human genomics, HiFi reads provide a high-resolution approach to study structural variants and their role in human health and disease. And with the recently launched Revio system bringing higher throughput and lower cost, HiFi data is opening a new era of genomic discovery.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
A new standard in repeat expansion detection & resolution of segmental duplications with HiFi reads PacBio 2023-03-28 | PacBio is changing the game of genomics with scalable and affordable, highly accurate long-reads from the Revio system. PacBio HiFi reads robustly detect the full spectrum of variation, moving beyond the limitations of other genomics technologies. In this workshop, speakers will share how HiFi sequencing can be applied to solve important clinical research problems such as fully resolving genes that lie within segmental duplications like SMN1/2 and detecting full-length repeat expansions.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Metagenome assembly and characterization of a pooled human fecal reference PacBio 2023-03-21 | Collectively, microorganisms represent one of the greatest biological forces on our planet — one that is both omnipotent and still largely unknown. To understand the multiplicity of influences that microbial communities have on everything from ecosystem function to human health, more sophisticated analytical tools are paramount. In this webinar, leading scientists from PacBio and Zymo Research present state-of-the art HiFi metagenomic sequencing solutions that will enable microbiome researchers to make important discoveries that are not only high-impact but more precise, reproducible, and resource-efficient than ever.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Tackling challenging genes and their paralogs involved in recessive disorders. PacBio 2023-02-22 | Effective carrier and newborn screening methods are critical for solving problems in reproductive medicine and launching treatment plans for early-onset genetic diseases. Clinical research faces a number of well-known challenges to disentangle the complexity of the underlying disease genetics. In this exciting webinar, PacBio scientists will showcase new HiFi sequencing solutions developed to accelerate high-impact discovery in carrier and newborn screening research, including Paraphase, a state-of-the-art software tool for analyzing SMN1/SMN2 and other genes with stunning accuracy.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
MAS-Seq for Single Cell Bioinformatics Walkthrough PacBio 2023-02-07 | The PacBio MAS-Seq for 10x Single Cell 3’ kit provides a 16-fold throughput increase on PacBio’s sequencing systems, enabling isoform resolution for single cell transcriptomics. In this tutorial, we demonstrate how to use PacBio’s SMRT Link software to analyze MAS-Seq single cell data to produce gene- and isoform-level count matrices that can be used with tertiary analysis tools such as Kana, Seurat, and CellTypist for cell clustering and cell labeling.For more information on PacBio’s single-cell isoform informatics, visit https://isoseq.howLearn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Iso-Seq for studying rAAV expression | Terrence Hanscome PacBio 2023-01-11 | Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
PacBio MAS-Seq TSO artifact removal demo | MAS-Seq for 10x Single Cell 3’ kit PacBio 2022-12-01 | The PacBio MAS-Seq for 10x Single Cell 3’ kit increases throughput by 16-fold for single-cell isoform sequencing on the PacBio systems. The TSO (template switching oligo) cDNA artifact removal step is demonstrated here in this video. To learn more about PacBio’s MAS-Seq method for single-cell isoform sequencing, visit pacb.com/products-and-services/applications/rna-sequencing/single-cell-rna-sequencingResearch use only. Not for use in diagnostic procedures. © 2022 Pacific Biosciences of California, Inc. (“PacBio”). All rights reserved. PacBio assumes no responsibility for any errors or omissions in this video. Certain notices, terms, conditions and/or use restrictions may pertain to your use of PacBio products and/or third-party products. Refer to the applicable PacBio terms and conditions of sale and to the applicable license terms at pacb.com/license.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Single-cell RNA sequencing with MAS-Seq. When genes are not enough. PacBio 2022-11-21 | During this engaging webinar, PacBio scientists present the MAS-Seq method, an all-new kitted single-cell transcriptomics solution that delivers unmatched data quality and explanatory power for your next big breakthrough discovery! Hear why isoforms, not genes, are critical for single-cell research. See how highly accurate, full-length isoform information at the single-cell level using the MAS-Seq method can deliver groundbreaking insights into disease biology. And finally, learn how to easily integrate MAS-Seq into your single-cell studies to discover hidden isoform diversity with a library prep walk-through and user-friendly informatics workflows.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
Unlock the next wave of discoveries in Human Genetics with PacBio technologies | ASHG 2022 CoLab PacBio 2022-11-16 | In this ASHG CoLab Theater presentation, Edd Lee, Director of Human Genomics Marketing, discusses key advances in PacBio technologies, including a new Tandom Repeat Genotyping Tool (TRGT) for mapping repeat expansions. He reviews PacBio's capabilities for HiFi amplicon sequencing and HiFi target enrichment. Edd also provides an overview of why the Human Pangenome Reference Consortium, Children's Mercy Research Institute, and Broad Institute have chosen HiFi sequencing as a fundamental technology to drive their human genomics research forward.In addition to Edd’s talk, you will also see a presentation from guest speaker Dr. Fritz Sedlazeck from Baylor College of Medicine entitled: Closing the gap: ‘Solving complex medically relevant regions of the human genome’. Dr. Sedlazeck discusses a panel he developed in collaboration with Twist Biosciences to assess critical medically relevant genes cost-effectively at large scale with applications across multiple diseases.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
The long and the short of it. Learn about the game changing capabilities of PacBio sequencing PacBio 2022-11-16 | CEO Christian Henry commences this action-packed workshop from ASHG 2022 with an overview of PacBio, including key milestones in the company's history. Dave Miller, VP of Product Marketing, then takes the podium and speaks about the evolution of Hifi sequencing and two new products: targeted HiFi sequencing at scale with off-the-shelf Twist panels and the new MAS-Seq for 10x Chromium Single Cell 3 Kit for high-throughput RNA isoform sequencing. He also discusses Sequencing By Binding (SBB) technology that has allowed PacBio to develop highly accurate, short-read capabilities. Dave then reveals new details about Onso, the new PacBio short-read sequencer. Dave also discusses test data that looks at Sequencing By Binding vs Sequencing By Synthesis for detecting rare variants. Dave then announces Revio, PacBio's new long-read sequencing system that delivers Hifi sequencing at scale. Finally, Dave speaks about Revio specs, as well as pricing, and shipping details for the two new sequencers. Dr. Stacey Gabriel from the Broad Institute then speaks on how Hifi sequencing is now an essential technology for increasing the completeness of genomic characterization of Biobank programs. She discusses expanding the data types that the All of Us Research Program is gathering, enabled by PacBio Hifi sequencing. Dr. Gabriel discusses the goals of adding long-reads to the All of Us data set and the strategies she is exploring to achieve the program's goals. She then announces that a pilot dataset is now available for researchers to explore and provides information on reproducible workflows for long-read processing at scale.To conclude the workshop, Dr. Aziz Al’Khafaji from the Broad Institute presents a fascinating talk on high-throughput RNA isoform sequencing with MAS-seq. Dr. Al’Khafaji discusses RNA isoforms and alternative splicing and explores how MAS-seq enables robust single-cell RNA isoform sequencing and resolves canonical CD45 splicing patterns. He wraps up by discussing key areas of application for MAS-seq and how the Broad’s Genomics Platform has productionized MAS-seq for researchers interested in submitting cDNA samples for the sequencing and data processing of a variety of library types.Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
The best keeps getting better — product portfolio updates from PacBio | ASHG 2022 CoLab PacBio 2022-11-16 | Join Aaron Wenger, PhD, for a deep dive into the updated PacBio product portfolio including the new Onso and the Revio Systems.00:00 - Introduction00:38 - HiFi Sequencing05:27 - HiFi Target Enrichment08:51 - MAS-Seq12:20 - Onso System13:07 - SBB Performance16:17 - Revio SystemLearn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
PacBio Product Launch Event at ASHG 2022. PacBio 2022-11-16 | Watch the Learn more about PacBio at pacb.comLegal & Trademarks: Visit pacb.com/legal-and-trademarks
