Preparing a Buffy Coat from Whole Blood @STEMCELLTechnologies

Uploaded October 2023 | Updated September 2026, 2 days ago

Generating a buffy coat from whole blood samples has several benefits, including concentrating large sample volumes and reducing downstream cell separation handling. This video demonstrates the washed-buffy coat procedure for generating a buffy coat from a whole blood sample while minimizing donor variability. The buffy coat can be used for downstream analyses or isolation of specific cell populations using immunomagnetic cell isolation technologies such as EasySep™.

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$How to Isolate Untouched Human NK Cells and Tips for High Recovery In this technical protocol video, you will learn how to isolate untouched human natural killer (NK) cells from a mononuclear cell suspension using the EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) and the The Big Easy EasySep™ Magnet (Catalog # 18001). The video includes optional steps to obtain higher NK cell recovery. This video is for demonstration purposes only; please consult the Product Information Sheet for further details on the protocol. EasySep™ Human NK Cell Isolation Kit (Catalog # 17955) https://www.stemcell.com/technical-resources/educational-materials/videos-and-webinars/how-to-isolate-human-regulatory-t-cells-tregs-using-the-easy-50-easysep-magnet.html?utm_source=youtube&utm_medium=link&utm_campaign=csi_immunology&utm_content=video_nkeasysephttps://www.stemcell.com/easysep-human-nk-cell-isolation-kit.html?utm_source=youtube&utm_medium=link&utm_campaign=cs_immunology&utm_content=nkvideo The Big Easy EasySep™ Magnet (Catalog # 18001) https://www.stemcell.com/products/the-big-easy-easysep-magnet.html Product Information Sheet: https://cdn.stemcell.com/media/files/pis/10000005299-PIS_01.pdf #EasySep #NaturalKillerCell #NKcell #NKcellisolation #CellSeparation #CellIsolation 0:00 - Intro 0:57 - Part I: Isolation of natural killer (NK) cells 2:39 - Addition of the EasySep™ Human NK Cell Isolation Cocktail (Catalog # 17955C) 4:12 - Addition of the EasySep™ Dextran RapidSpheres™ (Component # 50103) 6:13 - Top up of sample with EasySep™ Buffer (Catalog # 20144) and magnetic separation 7:04 - Collection of untouched NK cells 8:06 - Part II: Optional steps to achieve higher recovery 9:19 - Resuspension of EasySep™ Dextran RapidSpheres™ (Component # 50103) bound cell fraction and magnetic separation 11:14 - Collection of additional untouched NK cells 12:23 - Additional suggestion for higher recovery Below are more resources to learn how to prepare your samples for cell isolation: How to use SepMate™ to isolate peripheral blood mononuclear cells (PBMCs) from whole blood: https://www.youtube.com/watch?v=FM-7BwR0T9c How to recover cells from a leukocyte reduction system (LRS) cone: https://www.youtube.com/watch?v=k6vPSnJvudc How to process a leukopak for downstream cell isolation with EasySep™: https://www.stemcell.com/technical-resources/educational-materials/protocols/leukopak-processing-protocol.html Insights and techniques for efficient leukopak sample processing: https://www.stemcell.com/technical-resources/leukopak-processing-tips-and-tricks-for-streamlined-cell-isolation.html For a full list of products as well as educational resources, please visit our website: https://www.stemcell.com Follow us on Twitter: https://bit.ly/3jW20Gv Like us on Facebook: https://bit.ly/32ern07 Follow us on LinkedIn: https://bit.ly/32uKovJ Subscribe to our YouTube channel: https://bit.ly/32f28ei$

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Preparing a Buffy Coat from Whole Blood