Actin motility in dendritic spines Michael J Schell 2012-10-01 | Time-lapse widefield micrograph of a primary hippocampal neuron expressing F-Tractin-GFP, which highlights the dynamic actin within dendritic spines. Video playback is 180X real time
Glutamate uncaging initiates F-actin dynamics in a local cluster Michael J Schell 2015-10-28 | Primary hippocampal neurons expressing GFP fused to the F-actin reporter F-Tractin were stimulated at a single dendritic spine using the uncaging of glutamate. In this example, the activated spine exhibited massive cytoskeletal restructuring that developed over minutes, with delayed restructuring in a nearby spine. Confocal stacks were collected following uncaging, then deconvolved. Experiment done by Pavel Gusev.
Local collapse and regrowth of dendritic F-actin following glutamate Michael J Schell 2015-10-28 | Primary hippocampal cultures expressing F-Tractin-GFP to label cytoskeleton dynamics and tdTomato as a cytosolic marker. A strong 2-photon pulse at the location indicated by the arrow was applied to uncage glutamate locally in the bath. Confocal stacks collected and for about one hour after the stimulus then deconvolved. Experiment done by Pavel Gusev.
Photoswitching reveals actin turnover Michael J Schell 2015-10-28 | Cells expressing the actin reporter Lifeact fused to the photo-switchable protein Dendra2, which turns from green (left channel) to red (right channel) when excited with 405 nm light. Location indicated by arrow shows where the spot of the 405 laser was applied.
More dynamics of peroxisomes and mitochondira Michael J Schell 2012-10-02 | Time-lapse widefield movie showing C6 glioma cells expressing GFP-D-amino acid oxidase, which labels peroxisomes, and Ds-red targeted to mitochondira. The movie plays at 200X real time.
F-Tractin reports periodic F-actin waves Michael J Schell 2012-10-02 | Time-lapse a C6 glioma cell expressing F-Tractin fused to EGFP. Contrast has been inverted to highlight transient patches of actin polymers. Video plays at 200X real time.
GFP-actin in dendritic spines Michael J Schell 2012-10-02 | Time-lapse widefield micrograph of a hippocamal neuron (18 days in culture) expressing GFP-gamma Actin fusion protein. Video plays at 135X real time.
Actin-rich filopodia Michael J Schell 2012-10-02 | Time-lapse widefield micrograph of a C6 glioma cell expressing F-Tractin-GFP to visualize actin filaments. The movie plays at 200X real time.
Cell meets cell 2 Michael J Schell 2012-10-02 | Alternate presentation of Cell meets cell 1 video. Time-lape widefield micrographs of C6 glioma cells expressing F-Tractin-GFP to visualze F-actin dynamics. Top panel is an the fire LUT to highlight GFP fluorescence. Bottom panel is transmitted light with contrast enhanced with an emboss filter. Movie plays at 144X real time.
Cell meets cell 1 Michael J Schell 2012-10-02 | Time-lape widefield micrographs of C6 glioma cells expressing F-Tractin-GFP to visualze F-actin dynamics. Left panel is an inverted grayscale image of the GFP fluorescence. Right panel is transmitted light with contrast enhanced with an emboss filter. Movie plays at 144X real time.
Peroxisome dynamics Michael J Schell 2012-10-02 | Time-lapse widefield micrograph of C6 glioma cells expressing tdTomato-D-amino acid oxidase, which labels peroxisomes. The 3D effect was achieved with the emboss plugin uisng ImageJ. The movie plays at 3X real time.
Actin dynamics viewed with deconvolution microscopy Michael J Schell 2012-10-01 | Live deconvolution microscopy. This time-lapse movie depicts C6 glioma cells expressing F-Tractin-GFP, which labels actin filaments. The image on the right is a maximal projection of 5 widefield planes. The image on the right is a maximal projection of those planes following deconvolution using the Volocity rapid restoration routine. The movie plays at 240x real time.
Dynamics of peroxisomes and mitochondria Michael J Schell 2012-10-01 | Peroxisomes and mitochondria motility. Time-lapse widefield movie showing C6 glioma cells expressing GFP-D-amino acid oxidase, which labels peroxisomes, and Ds-red targeted to mitochondira. The movie plays at 24x real time.
Motochondria! Michael J Schell 2012-10-01 | Time-lapse widefield movie showing an axon-rich region of primary hippocampal cultures, which have been transfected with Ds-Red targeted to mitochondria. The movie plays at 60x real time.
Actin dynamics of lamellae Michael J Schell 2012-10-01 | 1. Time-lapse movie of a C6 glioma cell expressing F-Tractin-GFP. Z-stacks were acquired on a widefield microscope at 3 frames/minute, deconvolved using Volocity Rapid Restoration, and presented as maximal projections (total Z=distance of 0.8 µm). The movie plays at 200x real time.
F-actin accumulation in dendritic spines Michael J Schell 2012-10-01 | Time-lapse confocal micrograph of a cultured hippocampal neuron expressing GFP-ITPKA, which labels F-actin in dendritic spines. Video depicts a cell during a glutamate-free recovery period following a strong glutamatergic stimulus (100 micromolar glutamate for 20 seconds). The playback speed is 267X real time.
F-actin dynamics reported by F-Tractin Michael J Schell 2012-10-01 | Time lapse micrograph of a kidney precursor epithelial cell (7.1) expressing F-Tractin-GFP. Video plays at 120X real time.
Peroxisomes and Mitochondria Michael J Schell 2012-01-06 | Time lapse widefield microscopy of mitochondria labeled with red and peroxisomes labeled with green. The two organelles show complex interactions. Video plays at 48X real time.
NADH dynamics in mitochondria Michael J Schell 2012-01-06 | C6 glioma cells in culture, imaged by endogenous fluorescence for NADH, which occurs abundantly in mitochondria.
Dynamic actin filaments in dendritic spines Michael J Schell 2012-01-02 | Time-lapse widefield micrograph of a primary hippocamal neuron transfected with F-Tractin-EGFP to report F-actin dynamics in spines. Movie playback is at 312X real time.
Confocal vs 3D structured illumination Michael J Schell 2012-01-02 | Comparison of 3D renderings of synapses obtained with confocal microscopy (left) and 3D-SIM (Zeiss Elyra, right). Green is synaptic F-actin labeled with F-Tractin. Red is the active zone marker bassoon, labeled with an antibody.
Colorimetric visualization of the cell cycle Michael J Schell 2011-12-28 | Time-lapse movie of HeLa cells expressing the FUCCI cell cycle indicator. G1 phase is red, S, G2 and M phases are green. Video plays at 26,640X real time.
Motilty of ER and mitochondria Michael J Schell 2011-12-28 | HEK cells expressing GFP targeted to the endoplasmic reticulum (ER, green) and mito-tracker (red). Carbachol was added at point indicated, causing a temporary cessation in motilty.
