JAMESTOWN SETTLEMENT, Living History Museum MyGBLife 2024-10-11 | JAMESTOWN SETTLEMENT, Living History Museum
Calcium Carbonate Stability Test AKA Marble Test MyGBLife 2020-05-01 | Please be kind. I an trying to do the procedure and figuring out the best way to do it plus take video plus doing things with one hand and talk at the same time. My results are not the best. Just observe one possible way to do this procedure (My interpretation really, and not observing people in the field doing this procedure. I am a medical Lab person after all...)
Nitrate Using Nitrate Probe Millivolt Readings Plus Nitrate HACH Kit Testing MyGBLife 2020-04-03 | ...
Nitrite Test Using Spectrophotometer Method MyGBLife 2020-03-27 | Testing a drinking water sample for Nitrite using a spectrophotometer.Nitrite Using Spectrophotometer Method, rough draft, needs editing.Significance:Nitrite is significant is that ingestion of oxidizing agents such as nitrates or nitrites cause methemoglobinemia. Hemoglobin in human Red Blood cells is oxidized and converted to methemoglobin, and methemoglobin is unable to carry Oxygen through the blood stream. Nitrite is monitored and kept to less than 1mg/L.Principle:Nitrite combines with diazotized sulfanilamide with NED to make a reddish-purple azo dye at a pH of 2.0 to 2.5. This color is read spectrophotometrically. Absorbance is proportional to the amount of nitrite in the Test sample. This method is linear for samples up to 180ug/L N/L with a 1cm light path at 543nm.Specimen:Nitrite (NO2-) is unstable and wants to oxidize to nitrate. Because of instability, samples for nitrite should be tested immediately. If unable to test immediately, store samples up to 48 hours at 4 degrees Celsius to prevent bacterial conversion of Nitrite to Nitrate or Ammonia. Do not add acid to the Test sample or the nitrite will be converted to nitrate.Materials: 1. Nitrite free water (Assume our DI water is nitrite free, although DI water should be run through a demineralizer column to remove nitrites present.) 2. Color Reagent, prepared before class, containing phosphoric acid, sulfanilamide, and NED [N-(1-naphthyl)-ethylenediamine dihydrochloride] 3. Stock Nitrite solution, 100ppm. Make sure you use the bottle of standard labeled NITRITE and not NITRATE. It is different! 4. Spectrophotometer set up for 1 cm light path at 543 nm absorbance. The 5cm light path (rectangle cuvettes) are appropriate for Nitrate levels between 5 and 50ug N/L 5. pH paper. 6. Pipettors with tipsLimitations: 1. Concentrations need to be very small, so care must be used when preparing standards. Prepared standards only last one day. 2. Interferences of other ions may occur because they can cause precipitation: Sb3+, Au3+, Bi3+, Fe3+, Pb2+, Hg2+, Ag+, chloroplatinate (PtCl62+), and metavanadate (VO32-). 3. Cupric ions may cause false low results. 4. Colored ions may interfere with color reaction. 5. NCl3 may cause a false red color change when color reagent is added.Instrument verification:Spectrophotometer is verified by using spectrophotometer standards and method provided.Calibration:Spectrophotometer is calibrated at the factory.Quality Control: 1. A blank is used in the procedure. 2. If available a separate manufacturer control sample should be used to verify proper procedure is followed. 3. Nitrite stock solutions should be standardized. Our stock solution was standardized at the manufacturer.Procedure:Prepare calibrators: 1. Make a working standard by: A. Dilute the 100 mg/L Nitrite standard, 25 mL of 100mg/L standard in 100mL of DI water to make a 250ug/L solution. B. With that solution, dilute 50 mL in 250mL of DI water to make an intermediate solution of 5ug/L NO2-. C. With the intermediate solution, dilute 10mL in 1000mL of DI water to make a 0.5ug of NO2- working standard solution. 2. Prepare standards using 0.5ug working standard solution: (next page)Nitrite Standards - 50 mL volumetric flaskconcentration (ug/L as N)mL of 0.5ug/L Nitrite working sta 3. Add 2 mL of color reagent to 50 mL of the standards made. 4. Note: readings on the spec should be performed between 10 minutes and 2 hours following addition of color reagent. During this lag time, verify the spec is working properly (Verifying Photometric Accuracy, AKA Instrument Verification) 5. Produce calibration curve using the standards, absorbance versus ug/L as NProcedure, test sample: 1. Use filter flasks and 0.45um diameter pore membrane filter to filter your sample if sample appears turbid. 2. Determine the pH of your sample using pH paper. Record pH. The pH needs to be between 5 and 9 S.U. If you need to adjust the pH, use 1.0 N HCl or 1.0 N ammonium hydroxide. 3. Add 2.0 mL of the color solution to 50 mL of your test sample. 4. Reading on the spec should be performed between 10 minutes and 2 hours following addition of color reagent. 5. Determine nitrite concentration of test sample using calibration curve. If your Test sample has a nitrite level above 180ug/L, then dilute your sample so result is within that range.Calculations:Interpretation of Subjective Results: 1. If results did not go as planned, think about the dilutions of the 100mg/L standard. There are three dilutions of the standard being made, and with each dilution, a little more error is introduced. 2. Test sample must be in range of the standards, and free of particles. 3. Light path may need to be changed from 1 cm to 5 cm to get a good standard “curve” which really should be a straight line.Questions to test knowledge: 1. Why are parents
Culturing Organisms from Soil MyGBLife 2019-12-18 | Microbiology lab. Soil was weighed and several dilutions were made of the soil using dilution blanks. Then an amount of soil was added to 3 different types of plates to see what would grow using Spread Plate Technique.
Nisqually River Glacial River Mount Rainier National Park MyGBLife 2019-06-29 | This river is white in color because the water comes from a melting glacier. Glacial rivers are full of fine sediment, called glacial flour, created by the glacier grinding its rocky bed. The glacial flour gives the water a milky color.
Silver Falls Mount Rainier National Park Washington MyGBLife 2019-06-29 | This waterfall is near the Ohanapecosh Visitor Center. It is a fairly easy walk uphill compared to other waterfalls in the park. This water appears to be fresh water, compared to the rivers formed by glaciers.
Amnicon Falls State Park MyGBLife 2019-05-03 | Amnicon Falls is at a state park in Douglas County near Superior Wisconsin. There are three waterfalls here and a small campground. April 2019
Pattison State Park, Manitou Falls MyGBLife 2019-05-03 | Pattison State Park is in Northern Wisconsin, Douglas County, near Superior Wisconsin. The park has Big Manitou Falls, Little Manitou Falls, a beach, and a campground. Part of the park is closed due to high water. April 2019. At 165 feet, Big Manitou Falls is the highest waterfall in Wisconsin. Native Americans who settled nearby believed they heard the voice of the Great Spirit within the roaring of the falls and gave it the name "Gitchee Manitou"
Chlorine Demand, Chlorine ISE Probe MyGBLife 2019-03-16 | Another video, the best I can using one hand, video camera in one hand, and lots of concentration...but you get the idea, right?
Acid Rain Alaskan Pea Experiment MyGBLife 2018-12-07 | This is an experiment I set up for Biology class, where the students are looking at effects of pollution, like acid rain. I try to sprout peas in different acidic environments. You can see some of the mistakes I made and you can modify it for your own experiment. It almost seemed like a little acid sped up the sprouting, but why did the one dish have a lot of mold in it? Mold likes the acid? Lots of unanswered questions here....
Ammonia Probe Orizon VersaStar Assembly, Slope Test and Calibration MyGBLife 2018-09-29 | We are doing a "high Level" calibration for Ammonia using this High Performance Ammonia probe. Prepare the probe by replacing the membrane, run a slop test to check meter and probe function, calibrate, then run a test sample for quality control
Lab Prep Pour and Spread Plate Technique MyGBLife 2018-09-15 | This is my job to prepare media for the students for their "pour Plate" and "Spread Plate" labs. They will be making serial dilutions of a sample of E Coli in Brain Heart liquid Media, do their plating, and then the next week they will count their colonies and evaluate the differences between Pour plate and Spread plate.
Gram Stain Video, problem solving MyGBLife 2018-08-12 | There are many ways of making gram stain slides. Think about what is the ideal way to make slides and the ideal way to stain the slides. If the bacteria do not take up the stain well, what went wrong?
Streaking Plates, Microbiology MyGBLife 2018-08-12 | This style of streaking plates matches the lab manual in use. I plan on using this streaking style for consistency although the text books show different styles of streaked plates
Brimstone the Crested Gecko MyGBLife 2018-06-24 | Brim is a crested Gecko we got from a shelter. She was a surrender but is still very young. She is a sweetheart and very fun to play with, although she seems to always poop on the towel. She usually eats a lot of her food but seems a bit less hungry today
Buns the Cinnamon Ball Python MyGBLife 2018-06-23 | Buns is a cinnamon ball python wet got from a shelter. He was a rescue (hoarding situation). He is about 3 years old. He is still a little skittish but is getting better. This day was a day after his first shed at our house. It came off in two pieces so just a little was still on his skin.
Penn Plax Tortoise Palace Struggle But Together MyGBLife 2018-05-10 | Even though this tortoise pen had bad ratings and is way too expensive, I bought it for the window so I can see my tortoises as I sit at the dinner table.Now that I have it...it is not worth the cost. If you do not have an alternative, get someone skilled to help you put it together. It was a lot of work, but it is done now and I hope it lasts a long long time. I also really wish that company would care a little more about making a quality product and have some kind of customer service rather than pocketing huge profits from sales...OR I wish another company would design a nice habitat like it. There was also no reason why the glass was just wrapped in thin plastic wrap and not enclosed in strong cardboard. Very careless in this area.
Microscope Demo Olympus CX31 MyGBLife 2018-04-28 | beware some of my bad habits are showing through! Don't see them...
Death Valley National Park Points of Interest MyGBLife 2018-04-14 | Music by Kevin MacLeod Incompetech.com "Perspectives"
Deserts of Southern California Places to Visit MyGBLife 2018-04-14 | Music by Kevin MacLeod Incompetech,com "Americana"
Joshua Tree Naational Park, Desert of California MyGBLife 2018-04-14 | Music by Kevin MacLeod Incompetech.com "String Impromptu Number 1"
Fluoride Probe Set Up and Calibration, Nitrate Probe MyGBLife 2018-04-07 | Thermo Scientific Orion Versa Star Meters, Fluoride probes, Nitrate Probes, Direct calibration with standards, use plastic labware, slope check, stir plate. Fluoride probe set up and calibration and slope check very similar to the nitrate probe.
Antique Potato Planter Measurements MyGBLife 2018-04-01 | Just a closer look at my antique potato planter
HACH HQD Meters MyGBLife 2018-03-14 | Our HACH HQD meters used in class. I do not know much about the DO probe so must ask the teacher
Microman Pipettor MyGBLife 2018-03-04 | This is how to use these unusual pipettes for tiny microliter measurements
Manganese High Range Hach Test Kit Demo MyGBLife 2018-02-15 | Simple cook book directions. The only thing must ask the teacher about is the adjusting the sample to the proper pH to prevent the manganese from precipitating. The directions confused me.
Manganese Low Range Hach Kit demo MyGBLife 2018-02-15 | Simple cook book directions. The only thing must ask the teacher about is the adjusting the sample to the proper pH to prevent the manganese from precipitating. The directions confused me.
Total Hardness HACH Kit with Titrator testing MyGBLife 2018-02-07 | Please be kind. It is my first time trying this kit and only with one hand. Liquid volumes are measured at the meniscus
Measuring samples Spectrophotometer MyGBLife 2018-02-03 | First verify photometric accuracy before using. Genysis Vis spec
Verifying Photometric Accuracy Spectrophotometer MyGBLife 2018-02-03 | spectrophotometer verification using staandards
Calibrating pH Orion Versastar Meter MyGBLife 2018-02-03 | Calibration of the pH probe on the Orion Versastar meter. Process is similar to other pH meters. Here we are using pH standards 4, 7, and 10. Remember to re-cap the fill hole when finished.
Orion Versastar Meter Basic Use MyGBLife 2018-02-03 | Basic info on the meter we will use for pH, Conductivity, Chloride, Ammonia, Fluoride probes
Calibration Conductivity Probe MyGBLife 2018-02-03 | Orion Versastar Meter. Process is similar with the HQD Meters being used in Environmental Chemistry field sampling.
Divorced Single Woman Buys Real Christmas Tree All By Herself MyGBLife 2017-12-25 | Music by Kevin MacLeod Incompetech.com "We Wish You a Merry Christmas"It is a challenge being alone at times, especially buying a real live Christmas Tree. Many tree are on display so you can see what they look like. Since I cannot handle a displayed tree myself I found that I can just go to Menards and buy a tree that is still wrapped up. I don't know what it looks like, but I can put it in the cart and then into my car, and then into my house quite easily. I got at thinner tree on purpose this year. The trees have been very nice every year.
